RNA Sequencing:Article Title: Ethanolamine-induced assembly of microcompartments is required for Fusobacterium nucleatum virulence
Article Snippet: .. Table S1: RNA-seq analysis of the parent and eutV mutant strains grown in rich media Table S2: Mass spectrometry analysis of isolated BMCs Table S3: Bacterial strains and plasmids used in this study Strains & Plasmids Description Reference Strain F. nucleatum ATCC 23726 Type strain (5) F. nucleatum CW1 Derivative of 23726; lacking galK (5) F. nucleatum ∆eutN Isogenic derivative of CW1 lacking eutN This study F. nucleatum ∆eutL/∆eutM1/∆eutM2 Isogenic derivative of CW1 lacking eutL, eutM1, and eutM2 This study F. nucleatum ∆eutL/∆eutM1/∆eutM2/∆eutN Isogenic derivative of CW1 lacking eutL, eutM1, eutM2, and eutN This study F. nucleatum ∆eutV Isogenic derivative of CW1 lacking eutV This study Plasmid pCWU6 Derivative of pHS30 (5) pYWC1 Derivative of pCWU6 This study pCM-GalK C. perfringens vector expressing galK (5) p∆eutN pCM-galK derivative; eutN deletion vector This study p∆eutLM1M2 pCM-galK derivative; eutLM1M2 deletion vector This study p∆eutV pCM-galK derivative; eutV deletion vector This study pEutN Derivative of pYWC1 expressing eutN under the control of a RsmA promoter This study 4 Table S4: Primers used in this study ..
Mutagenesis:Article Title: Ethanolamine-induced assembly of microcompartments is required for Fusobacterium nucleatum virulence
Article Snippet: .. Table S1: RNA-seq analysis of the parent and eutV mutant strains grown in rich media Table S2: Mass spectrometry analysis of isolated BMCs Table S3: Bacterial strains and plasmids used in this study Strains & Plasmids Description Reference Strain F. nucleatum ATCC 23726 Type strain (5) F. nucleatum CW1 Derivative of 23726; lacking galK (5) F. nucleatum ∆eutN Isogenic derivative of CW1 lacking eutN This study F. nucleatum ∆eutL/∆eutM1/∆eutM2 Isogenic derivative of CW1 lacking eutL, eutM1, and eutM2 This study F. nucleatum ∆eutL/∆eutM1/∆eutM2/∆eutN Isogenic derivative of CW1 lacking eutL, eutM1, eutM2, and eutN This study F. nucleatum ∆eutV Isogenic derivative of CW1 lacking eutV This study Plasmid pCWU6 Derivative of pHS30 (5) pYWC1 Derivative of pCWU6 This study pCM-GalK C. perfringens vector expressing galK (5) p∆eutN pCM-galK derivative; eutN deletion vector This study p∆eutLM1M2 pCM-galK derivative; eutLM1M2 deletion vector This study p∆eutV pCM-galK derivative; eutV deletion vector This study pEutN Derivative of pYWC1 expressing eutN under the control of a RsmA promoter This study 4 Table S4: Primers used in this study ..
Mass Spectrometry:Article Title: Ethanolamine-induced assembly of microcompartments is required for Fusobacterium nucleatum virulence
Article Snippet: .. Table S1: RNA-seq analysis of the parent and eutV mutant strains grown in rich media Table S2: Mass spectrometry analysis of isolated BMCs Table S3: Bacterial strains and plasmids used in this study Strains & Plasmids Description Reference Strain F. nucleatum ATCC 23726 Type strain (5) F. nucleatum CW1 Derivative of 23726; lacking galK (5) F. nucleatum ∆eutN Isogenic derivative of CW1 lacking eutN This study F. nucleatum ∆eutL/∆eutM1/∆eutM2 Isogenic derivative of CW1 lacking eutL, eutM1, and eutM2 This study F. nucleatum ∆eutL/∆eutM1/∆eutM2/∆eutN Isogenic derivative of CW1 lacking eutL, eutM1, eutM2, and eutN This study F. nucleatum ∆eutV Isogenic derivative of CW1 lacking eutV This study Plasmid pCWU6 Derivative of pHS30 (5) pYWC1 Derivative of pCWU6 This study pCM-GalK C. perfringens vector expressing galK (5) p∆eutN pCM-galK derivative; eutN deletion vector This study p∆eutLM1M2 pCM-galK derivative; eutLM1M2 deletion vector This study p∆eutV pCM-galK derivative; eutV deletion vector This study pEutN Derivative of pYWC1 expressing eutN under the control of a RsmA promoter This study 4 Table S4: Primers used in this study ..
Isolation:Article Title: Ethanolamine-induced assembly of microcompartments is required for Fusobacterium nucleatum virulence
Article Snippet: .. Table S1: RNA-seq analysis of the parent and eutV mutant strains grown in rich media Table S2: Mass spectrometry analysis of isolated BMCs Table S3: Bacterial strains and plasmids used in this study Strains & Plasmids Description Reference Strain F. nucleatum ATCC 23726 Type strain (5) F. nucleatum CW1 Derivative of 23726; lacking galK (5) F. nucleatum ∆eutN Isogenic derivative of CW1 lacking eutN This study F. nucleatum ∆eutL/∆eutM1/∆eutM2 Isogenic derivative of CW1 lacking eutL, eutM1, and eutM2 This study F. nucleatum ∆eutL/∆eutM1/∆eutM2/∆eutN Isogenic derivative of CW1 lacking eutL, eutM1, eutM2, and eutN This study F. nucleatum ∆eutV Isogenic derivative of CW1 lacking eutV This study Plasmid pCWU6 Derivative of pHS30 (5) pYWC1 Derivative of pCWU6 This study pCM-GalK C. perfringens vector expressing galK (5) p∆eutN pCM-galK derivative; eutN deletion vector This study p∆eutLM1M2 pCM-galK derivative; eutLM1M2 deletion vector This study p∆eutV pCM-galK derivative; eutV deletion vector This study pEutN Derivative of pYWC1 expressing eutN under the control of a RsmA promoter This study 4 Table S4: Primers used in this study ..
Plasmid Preparation:Article Title: Ethanolamine-induced assembly of microcompartments is required for Fusobacterium nucleatum virulence
Article Snippet: .. Table S1: RNA-seq analysis of the parent and eutV mutant strains grown in rich media Table S2: Mass spectrometry analysis of isolated BMCs Table S3: Bacterial strains and plasmids used in this study Strains & Plasmids Description Reference Strain F. nucleatum ATCC 23726 Type strain (5) F. nucleatum CW1 Derivative of 23726; lacking galK (5) F. nucleatum ∆eutN Isogenic derivative of CW1 lacking eutN This study F. nucleatum ∆eutL/∆eutM1/∆eutM2 Isogenic derivative of CW1 lacking eutL, eutM1, and eutM2 This study F. nucleatum ∆eutL/∆eutM1/∆eutM2/∆eutN Isogenic derivative of CW1 lacking eutL, eutM1, eutM2, and eutN This study F. nucleatum ∆eutV Isogenic derivative of CW1 lacking eutV This study Plasmid pCWU6 Derivative of pHS30 (5) pYWC1 Derivative of pCWU6 This study pCM-GalK C. perfringens vector expressing galK (5) p∆eutN pCM-galK derivative; eutN deletion vector This study p∆eutLM1M2 pCM-galK derivative; eutLM1M2 deletion vector This study p∆eutV pCM-galK derivative; eutV deletion vector This study pEutN Derivative of pYWC1 expressing eutN under the control of a RsmA promoter This study 4 Table S4: Primers used in this study ..
Expressing:Article Title: Ethanolamine-induced assembly of microcompartments is required for Fusobacterium nucleatum virulence
Article Snippet: .. Table S1: RNA-seq analysis of the parent and eutV mutant strains grown in rich media Table S2: Mass spectrometry analysis of isolated BMCs Table S3: Bacterial strains and plasmids used in this study Strains & Plasmids Description Reference Strain F. nucleatum ATCC 23726 Type strain (5) F. nucleatum CW1 Derivative of 23726; lacking galK (5) F. nucleatum ∆eutN Isogenic derivative of CW1 lacking eutN This study F. nucleatum ∆eutL/∆eutM1/∆eutM2 Isogenic derivative of CW1 lacking eutL, eutM1, and eutM2 This study F. nucleatum ∆eutL/∆eutM1/∆eutM2/∆eutN Isogenic derivative of CW1 lacking eutL, eutM1, eutM2, and eutN This study F. nucleatum ∆eutV Isogenic derivative of CW1 lacking eutV This study Plasmid pCWU6 Derivative of pHS30 (5) pYWC1 Derivative of pCWU6 This study pCM-GalK C. perfringens vector expressing galK (5) p∆eutN pCM-galK derivative; eutN deletion vector This study p∆eutLM1M2 pCM-galK derivative; eutLM1M2 deletion vector This study p∆eutV pCM-galK derivative; eutV deletion vector This study pEutN Derivative of pYWC1 expressing eutN under the control of a RsmA promoter This study 4 Table S4: Primers used in this study ..
Control:Article Title: Ethanolamine-induced assembly of microcompartments is required for Fusobacterium nucleatum virulence
Article Snippet: .. Table S1: RNA-seq analysis of the parent and eutV mutant strains grown in rich media Table S2: Mass spectrometry analysis of isolated BMCs Table S3: Bacterial strains and plasmids used in this study Strains & Plasmids Description Reference Strain F. nucleatum ATCC 23726 Type strain (5) F. nucleatum CW1 Derivative of 23726; lacking galK (5) F. nucleatum ∆eutN Isogenic derivative of CW1 lacking eutN This study F. nucleatum ∆eutL/∆eutM1/∆eutM2 Isogenic derivative of CW1 lacking eutL, eutM1, and eutM2 This study F. nucleatum ∆eutL/∆eutM1/∆eutM2/∆eutN Isogenic derivative of CW1 lacking eutL, eutM1, eutM2, and eutN This study F. nucleatum ∆eutV Isogenic derivative of CW1 lacking eutV This study Plasmid pCWU6 Derivative of pHS30 (5) pYWC1 Derivative of pCWU6 This study pCM-GalK C. perfringens vector expressing galK (5) p∆eutN pCM-galK derivative; eutN deletion vector This study p∆eutLM1M2 pCM-galK derivative; eutLM1M2 deletion vector This study p∆eutV pCM-galK derivative; eutV deletion vector This study pEutN Derivative of pYWC1 expressing eutN under the control of a RsmA promoter This study 4 Table S4: Primers used in this study ..
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